Input Instructions

Download Sample Input

The input file must be a tab-delimited text file no larger than 5Mb with the following five columns:

  1. Experiment ID
  2. Replicate
  3. Bait
  4. Prey
  5. Spectral Count

Input Field Details

Experiment ID
A unique ID for a given AP-MS experiment. Replicates will all share the same Experiment ID
Replicate
An ID for the replicate in a given experiment
Experiment Type
This field is ignored and is allowed for backward compatability with the Spotlite website.
Bait
The ID for the bait used in the experiment. This is usually the gene symbol.
Prey
The ID for the prey found in the experiment. This is also usually the gene symbol. This ID type should match that used for the Bait.
Spectral Count
The number of spectra found per bait-prey pair in an experimental replicate.

Output Description

Experiment.ID
The unique ID for a given AP-MS experiment provided by the input
Bait
The unique ID for the bait in the AP-MS experiment provided by the input
Prey
The unique ID for the prey in the AP-MS experiment provided by the input
AvePSM
The mean spectral counts across all the replicates in an experiment provided by the input
Z
The Z-Score for the prey given all other experiments provided.
WD
The WD score defined by Sowa et al.
Entropy
The Shannon Entropy for each spectral count provided by the input