Collaboration

A Gygi & Harper Lab Collaboration

Funding

Biogen

NHGRI: U41 HG006673

Google Ventures/Third Rock Ventures

Clone Availability

All lentiviral expression constructs used in this study are available upon request. Contact Laura Pontano Vaites and Wade Harper for assistance.

Primary BioPlex References:

BioPlex 1.0: Huttlin EL, Ting L, Bruckner RJ, Gebreab F, Gygi MP, Szpyt J, Tam S, Zarraga G, Colby G, Baltier K, Dong R, Guarani V, Vaites LP, Ordureau A, Rad R, Erickson BK, Wuhr M, Chick J, Zhai B, Kolippakkam D, Mintseris J, Obar RA, Harris T, Artavanis-Tsakonas S, Sowa ME, De Camilli P, Paulo JA, Harper JW, Gygi SP. (2015) The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 162:425-440. Github

BioPlex 2.0: Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW. (2017) Architecture of the Human Interactome Defines Protein Communities and Disease Networks. Nature 545:505-509. Github

BioPlex 3.0: Huttlin EL, Bruckner RJ, Navarrete-Perea J, Cannon JR, Baltier K, Gebreab F, Gygi MP, Thornock A, Zarraga G, Tam S, Szpyt J, Gassaway BM, Panov A, Parzen H, Fu S, Golbazi A, Maenpaa E, Stricker K, Guha Thakurta S, Zhang T, Rad R, Pan J, Nusinow DP, Paulo JA, Schweppe DK, Pontano Vaites L, Harper JW, Gygi SP. (2021) Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome. Cell 184(11):3022-3040.e28. doi:10.1016/j.cell.2021.04.011. Github

Other BioPlex References:

BioPlex Display & Website: Schweppe DK, Huttlin EL, Harper JW, Gygi SP. (2017) BioPlex Display: An Interactive Suite for Large-scale, AP-MS Protein-protein Interaction Data. J. Proteome Res. doi: 10.1021/acs.jproteome.7b00572.

Preprints:

BioPlex 3.0: Huttlin EL, Bruckner RJ, Navarrete-Perea J, Cannon JR, Baltier K, Gebreab F, Gygi MP, Thornock A, Zarraga G, Tam S, Szpyt J, Panov A, Parzen H, Fu S, Golbazi A, Maenpaa E, Stricker K, Guha Thakurta S, Rad R, Pan J, Nusinow DP, Paulo JA, Schweppe DK, Pontano Vaites L, Harper JW, Gygi SP. (2020) Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome. bioRxiv doi:10.1101/2020.01.19.905109 Github

Mapping Cell Structures with BioPlex and Human Protein Atlas: Qin Y, Winsnes CF, Huttlin EL, Zheng F, Ouyang W, Park J, Pitea A, Kreisberg JF, Gygi SP, Harper JW, Ma J, Lundberg E, Ideker T. (2020) Mapping Cell Structure across Scales by Fusing Protein Images and Interactions. bioRxiv doi:10.1101/2020.06.21.163709 Github

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